A Simple Way to Multiplex PCR with Wax Films

Have you ever wanted to test for multiple diseases or genetic conditions in one go? Scientists often use a technique called multiplex PCR to achieve this. But the equipment needed for this is complicated and tough to handle. A recent study introduced a clever way to make this process simpler using wax films. Imagine having tiny cups for each PCR reaction on a small chip. This chip has 24 of these tiny reaction chambers, all connected by a single channel. At the bottom of each chamber is a wax film that seals the chambers tightly. This wax film keeps the special chemicals, called primers, from mixing with the reaction fluids until just the right time.When the chip is placed in a thermal cycler, the wax films melt during the PCR process, releasing the primers at the perfect moment. This makes everything work together seamlessly. To test this new method, scientists used it to detect seven common mutations related to deafness. The results matched with those from a standard test called Sanger sequencing. This shows that the new method works well and can be used in real-life situations.