Growing Epithelial Cells with Invasin: A New Way to Expand Organoids

Ever wondered how to keep epithelial cells growing for a long time? Scientists found that a protein called Invasin, produced by a type of bacteria called Yersinia, can help. This protein works by activating something called integrin, which helps cells stick together and grow. When integrated into a culture system, Invasin allowed gut epithelial cells to grow as flat sheets, or 2D organoids. Matrigel, a substance that mimics basement membranes, is usually used to help epithelial cells grow in 3D. But it has its downsides. Invasin offers a simpler, more affordable alternative. It allows cells to stick to the culture plate and grow in a flatter, more accessible way. This makes it easier to study and image the cells.The best part? Invasin works with different types of epithelial cells, from humans to mice and even snakes. It supports the growth of various cell types, like enterocytes, goblet cells, and enteroendocrine cells. This means scientists can study how these cells behave and interact over time. Using Invasin, scientists can now expand epithelial cells more easily and affordably. This could open up new ways to study diseases, test drugs, and understand how our bodies work.