How Fixing Tissues Right Matters in Animal Health Studies
In the world of animal health research, the handling of tissues plays a pivotal role. Fixation, the process of preserving tissues, significantly influences their appearance under a microscope and their performance in molecular tests.
The Challenges with Unbuffered Formalin
A recent study highlighted the issues associated with unbuffered formalin, a commonly used preservative. The findings revealed that:
- Cells appear more spread out
- Color contrast in stains is reduced
- DNA analysis becomes more challenging
These issues are critical because high-quality tissue samples are essential for accurate diagnosis and research.
The Problems of Over-Fixation
The study also identified that over-fixation can lead to:
- Weakened signals in tests detecting specific proteins
- More broken-up DNA
Simple Solutions for Better Tissue Handling
Fortunately, the researchers discovered straightforward solutions:
- Storing tissues in ethanol after fixation helps maintain strong protein signals.
- Coating tissue blocks with extra paraffin protects DNA from degradation over time.
Best Practices for Tissue Handling
The study recommends:
- Using buffered formalin
- Fixing tissues within 72 hours
These practices ensure optimal results for both microscopic examination and molecular testing.
The Importance of Standardized Tissue Handling
High-quality tissue samples are crucial for precision diagnostics. They enable veterinarians and researchers to:
- Better understand diseases
- Develop more effective treatments
Standardizing tissue handling is not just about adhering to protocols; it's about enhancing animal healthcare.