MicroRNAs and Ovarian Function: A Computational Journey

Ever wondered how tiny molecules can influence big issues like infertility? Meet microRNAs (miRNAs), which are gaining attention for their role in Diminished Ovarian Reserve (DOR), a major cause of infertility. Scientists are diving into the world of miRNAs to understand how they affect granulosa cells and follicular fluid, key players in follicle development. Using a computational approach, researchers created a network called FGC_MiRNome_MC, representing miRNA target genes and their interactions. They found 365 crucial hubs within this network, which act like important regulators. Among these, 210 nodes were identified as both hubs and bottlenecks (H&BN nodes), suggesting they control information flow.By analyzing these H&BN nodes, researchers discovered they play a part in essential cellular processes linked to ovarian function. Notably, the RAS/MAPK pathway, vital for cell proliferation, differentiation, and survival, was identified. This pathway could be a target for further investigation to understand DOR better. Interestingly, these hub and bottleneck genes were also connected to reproductive health in mouse models. This computational journey offers valuable insights into potential biomarkers and therapeutic targets for age-related reproductive disorders.