Testing for COVID-19 in Air: New Methods Compared

You're in a room filled with tiny airborne particles. Some of these might be carrying the SARS-CoV-2 virus, the cause of COVID-19. Scientists recently tried to figure out the best way to detect this virus in the air we breathe. They tested three different methods: quantitative polymerase chain reaction (qPCR), digital droplet polymerase chain reaction (ddPCR), and loop-mediated isothermal amplification (LAMP). First, they simulated how a person breathes and collected the airborne virus particles, called bioaerosols. Then, they tested how well these three methods could find the virus in these particles. They also made sure the tools for LAMP were really good at finding only SARS-CoV-2 and not mix-ups.To do this, they created an environment where they could control the amount and type of virus particles. They used a special machine to collect these particles from the air. After collecting, they compared how well each method could detect the virus in both the air and a liquid solution. What they found was interesting: qPCR was a bit better at finding the virus in liquid, but not as good with tiny amounts in the air. LAMP could tell if the virus was there or not, while ddPCR could give an exact count, even with very small amounts. The best part? These methods could be used in places like hospitals or crowded areas to quickly find small amounts of virus in the air. This could give us an early warning when the virus is around, helping to protect people.