Watching Worms: How Ivermectin Resistance Changes with Drug Dose

You're running a massive experiment with tiny worms. You're studying how they evolve resistance to a drug called ivermectin. To do this, you use a cool new tool called TARDIS barcoding. This tool helps you track the worms' genetic lineage, just like a family tree. Why worms? Because they're easy to study and they have something called GluCl channels. These channels make worms sensitive to ivermectin. When you knock out three of these channels, the worms become resistant to high doses of the drug.Now, you grow these worms in liquid culture and add different amounts of ivermectin. This lets you control how strong the drug's effect is. You can then see how well the resistant worms do compared to the normal ones. What happens? At low doses, the resistant worms struggle. They don't grow as fast and quickly lose out to the normal worms. But at high doses, the tables turn. The resistant worms thrive, showing that drug resistance has a cost. Why is this important? Well, it's not just about worms. Understanding how drug resistance works can help us in the fight against diseases that affect both humans and animals.