Hyaluronic acid (HA) is a big deal in the world of skincare and medicine. But making it in a cost-effective way is still a challenge. Scientists have been experimenting with different temperatures and pH levels to find the best conditions for producing HA using bacteria.

They tested four temperatures: 32°C, 35°C, 37°C, and 40°C. They also tested four pH levels: 6. 5, 7. 0, 7. 5, and 8. 0. The goal was to find the perfect mix for the highest yield.

Using a method called the Taguchi method, they found that 37°C and pH 6. 5 gave the best results. This combo produced the most HA, about 1. 08 grams per liter. The Taguchi method helped them see which conditions worked best by looking at the signal-to-noise ratios.

The scientists also found that 37°C was way better than 32°C and 40°C for making HA. At 40°C, the bacteria didn't make much HA at all. But at 32°C, the amount of HA didn't change much, which is interesting.

They also noticed that the more bacteria there were, the more HA they made. The best bacterial growth happened at 37°C and pH 7. 0. This shows that the conditions for making HA and growing bacteria are closely linked.

This study gives important clues about how to make HA more efficiently. It shows that small changes in temperature and pH can make a big difference in how much HA is produced.

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