Alzheimer's disease is a growing concern, with millions of people affected worldwide. Early detection is crucial for better management and treatment. Two important indicators of this disease are monoamine oxidase B (MAO-B) and acetylcholinesterase (AChE). These substances are often elevated in individuals with Alzheimer's. However, finding both of these indicators at the same time is quite challenging. A recent development has changed the game. A new method has been created to spot both MAO-B and AChE simultaneously. This breakthrough uses a special sensor based on a one-pot reaction system. This system is more efficient and provides more detailed information for early diagnosis.

The sensor works by using gold nanoparticles (Au NPs) modified with a Raman reporter called 4-aminothiophenol (AuPATP NPs). These nanoparticles are attached to a substance called Cu-BTC through a chemical reaction known as Schiff's base reaction. This process results in a strong Raman signal, which is a type of light scattering used for detection. The sensor's clever design allows it to detect both MAO-B and AChE at the same time.

Here's how it works: Phenethylamine (PEA), a substance that MAO-B acts upon, competes with the AuPATP NPs for binding sites on Cu-BTC. This competition reduces the Raman signal intensity, indicating the presence of MAO-B. Additionally, two PATP molecules on free Au NPs couple to form DMAB due to the catalysis of Cu2+ in Cu-BTC. This transformation generates new Raman peaks, further aiding in detection. For AChE, the catalytic product TCh chelates with Cu2+, decreasing the coupling efficiency of PATP. This prevents the conversion of AuPATP NPs to AuDMAB NPs, providing another signal for detection.

The sensor's detection limits are impressively low, at 2. 3 x 10^-3 micrograms per milliliter for MAO-B and 1. 6 x 10^-3 units per liter for AChE. This high sensitivity is crucial for early detection. The method was successfully tested in serum samples, with recovery rates ranging from 100. 0 to 113. 7% for MAO-B and 93. 6 to 120% for AChE. These results show the sensor's potential for real-world applications. The ability to detect both biomarkers simultaneously is a significant step forward. It offers a more comprehensive approach to Alzheimer's diagnosis, potentially leading to earlier interventions and better patient outcomes.

This new method is a game-changer in the field of Alzheimer's research. It provides a more efficient and effective way to detect key biomarkers, paving the way for better diagnosis and treatment. As research continues, this sensor could play a crucial role in the fight against Alzheimer's disease.

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